Sains Malaysiana 52(9)(2023): 2599-2612

http://doi.org/10.17576/jsm-2023-5209-10

Volatile Compounds Detected by SPME-GC-MS in Fish Viscera Extracts with Attractant Activity against House Fly Musca domesticafrom Chemometric FTIR Fingerprints

(Sebatian Meruap Dikesan oleh SPME-GC-MS dalam Ekstrak Visera Ikan dengan Aktiviti Bahan Penarik terhadap Lalat Rumah Musca domestica daripada Cap Jari FTIR Kimometriks)

RAHMATIA GARWAN^1,4,5, HANIFAH NURYANI LIOE^1,*, TATI NURHAYATI², NANCY DEWI YULIANA¹, SUPRIYONO ³ & HARSI DEWANTARI KUSUMANINGRUM¹

¹Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, IPB University, Bogor 16680, Indonesia

 ²Department of Fishery Products Technology, Faculty of Fisheries and Marine Sciences, IPB University, Bogor 16680, Indonesia

³Department of Animal Disease Science and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia

⁴Food Agency of the Local Government of North Moluccas Province, Indonesia

⁵Faculty of Agriculture, Muhammadiyah University of North Moluccas, Indonesia

Diserahkan: 23 April 2023/Diterima: 17 Ogos 2023

Abstract

Fish viscera has been locally applied as fly attractant in North Moluccas. The objective of this study was to evaluate the efficacy of attractant from fish viscera extracts and to determine the indispensable active extracts with chemometric FTIR fingerprints, as well as to characterize volatile compounds of the extracts. Attractant activity against house fly Musca domesctica and FTIR spectra were analyzed for the ethanol, acetone and hexane extracts of stomach, intestines, liver, and combination of the viscera from skipjack tuna stored for 1-2 days at room temperature. SPME-GC-MS analysis was applied for active extracts to determine volatile compounds. OPLS analysis result showed that FTIR spectra were correlated with the attractant activity (R² 0.974 and Q² 0.89), and compounds with functional groups S-H, N-H, O-H, and C-O have the strong correlation. Ethanol and acetone extracts of mixed viscera from 2 days storage are the active extracts. The volatile compounds in the extract through the SPME-GC-MS analysis belong to ketone, amine, phenol, heterocyclic, aromatic aldehyde, fatty acid, fatty acid ester and sulfur compounds are reported.

Keywords: Attractant activity; fish viscera extracts; Musca domestica; chemometric; FTIR fingerprint; SPME-GC-MS

Abstrak

Visera ikan telah digunakan secara tempatan sebagai bahan penarik lalat di Maluku Utara. Objektif kajian ini adalah untuk menilai keberkesanan bahan penarik daripada ekstrak visera ikan dan menentukan ekstrak aktif yang sangat diperlukan dengan cap jari FTIR kimometriks selain mencirikan sebatian meruap bagi ekstrak tersebut. Aktiviti bahan penarik terhadap lalat rumah Musca domesctica dan spektrum FTIR dianalisis untuk ekstrak etanol, aseton dan heksana perut, usus, hati dan gabungan visera daripada tuna cakalang yang disimpan selama 1-2 hari pada suhu bilik. Analisis SPME-GC-MS digunakan untuk ekstrak aktif dalam menentukan sebatian meruap. Keputusan analisis OPLS menunjukkan bahawa spektrum FTIR berkorelasi dengan aktiviti bahan penarik (R² 0.974 dan Q² 0.89) serta sebatian kumpulan berfungsi S-H, N-H, O-H dan C-O mempunyai korelasi yang kuat. Ekstrak etanol dan aseton daripada gabungan visera daripada simpanan 2 hari adalah merupakan ekstrak aktif. Sebatian meruap dalam ekstrak melalui analisis SPME-GC-MS dilaporkan tergolong kepada keton, amina, fenol, heterosiklik, aldehid aromatik, asid lemak, ester asid lemak dan sebatian sulfur.

Kata kunci: Aktiviti bahan penarik; cap jari FTIR; ekstrak visera ikan; kimometriks; Musca domestica; SPME-GC-MS

RUJUKAN

Abbott, W.S. 1925. A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18: 265-267.

Al-Mesbah, H., Moffatt, C., El-Azazy, O.M. & Majeed, Q.A. 2012. The decomposition of rabbit carcasses and associated necrophagous Diptera in Kuwait. Forensic Science International 217: 27-31.

AOAC. 2012. Official Methods of Analysis of AOAC International. 19th ed. Maryland: AOAC International Press. pp. 27-29.

Balino-Zuazo, L. & Barranco, A. 2016. A novel liquid chromatography-mass spectrometric method for the simultaneous determination of trimethylamine, dimethylamine and methylamine in fishery products. Food Chemistry 196: 1207-1214. 

Bourigua, S., El Ichi, S., Korri-Youssoufi, H., Maaref, A., Dzyadevych, S. & Renault, N.J. 2011. Electrochemical sensing of trimethylamine based on polypyrrole–flavin-containing monooxygenase (FMO3) and ferrocene as redox probe for evaluation of fish freshness. Biosensors and Bioelectronics 28: 105-111.

Castañeda-Pérez, E., Osorio-Revilla, G.I., Gallardo-Velázquez, T. & Proal-Nájera, J.B. 2013. Use of FTIR-HATR coupled to multivariate analysis to monitor the degradation of bioactive compounds during drying of red pepper. Revista Mexicana De Ingeniería Química 12: 193-204.

Chang, Y., Hou, H. & Bafang, L. 2016. Identification of volatile compounds in codfish (Gadus) by a combinationof two extraction methods coupled with GC-MS analysis. Journal of Ocean University of China 15: 509-514.

Cheng, H., Mei, J. & Xie, J. 2023. Analysis of key volatile compounds and quality properties of tilapia (Oreochromis mossambicus) fillets during cold storage: Based on thermal desorption coupled with gas chromatography-mass spectrometry (TD-GC-MS). Food Science and Technology 184: 115051.

Chin, H.C., Kurahashi, H., Marwi, MA., Jeffery, J. & Omar, B. 2011. Opportunistic insects associated with pig carrions in Malaysia. Sains Malaysiana 40(6): 601-604.

 Dehaut, A., Krzewinski, F., Grard, T., Chollet, M., Jacques, P., Brisabois, A. & Duflos, G. 2016. Monitoring the freshness of fish: development of a qPCR method applied to MAP chilled whiting. Journal of the Science of Food and Agriculture 96: 2080-2089.

Duflos, G., Leduc, F., N'Guessan, A., Krzewinski, F., Kol, O. & Malle, P. 2010. Freshness characterization of whiting (Merlangius merlangus) using SPME/GC/MS method and a statistical multivariate approach. Journal of the Science of Food and Agriculture 90: 2568-2575.

Erickson, M.C., Ma, L.M. & Doyle, M.P. 2015. Development of models to relate microbiology and headspace volatile parameters in stored Atlantic salmon to acceptance and willingness to prepare the product by senior consumers. Journal of Food Protection 78(12): 2156-2169.

Francioso, F., Conrado, A.B., Mosca, L. & Fontana, M. 2020. Chemistry and biochemistry of sulfur natural compounds: Key intermediates of metabolism and redox biology. Oxidative Medicine and Cellular Longevity 2020: 8294158.

Garwan, R., Kusumaningrum, H.D., Nurhayati, T. & Lioe, H.N. 2022. Karakterisasi jeroan ikan cakalang sebagai skrining awal bahan baku perangkap lalat rumah M. domesticadan antibakteri. Jurnal Pengolahan Hasil Perikanan Indonesia 25(1): 34-51.

Harborne, J.B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Terbitan kedua, translated by Padmawinata, K. & Soediro, I. Bandung: Penerbit ITB.

He, R., Yao, X.Z., Chen, M., Ma, R.C., Li, H.J., Wang, C. & Ding, S.H. 2018. Conversion of sulfur compounds and microbial community in anaerobic treatment of fish and pork waste. Waste Management 76: 383-393.

Hou, H., Zhao, X., Li, B., Li, P., Zhang, Z., Shao, X., Pang, W. & Qu, X. 2013. Solid-phase microextraction method for the determination of volatile compounds in hydrolisates of Alaska Pollock frame. International Journal of Food Properties 16: 790-802.

Ilza, M. & Diharmi, A. 2022. Processing and utilization of natural phospholipids from fish belly fat waste of jambal siam fish (Pangasius hypophthalamus). In IOP Conference Series: Earth and Environmental Science 1118(1): 012041.

Kun, Q., Zhu, J.J., Sims, S.R., Taylor, D.B. & Zeng, X. 2013. Identification of volatile compounds from a food-grade vinegar attractive to house flies (Diptera: Muscidae). Journal of Economic Entomology 106(2): 979-987.

Leduc, F., Krzewinski, F., Le Fur, B., N'Guessan, A., Malle, P., Kol, O. & Duflos, G. 2012. Differentiation of fresh and frozen/thawed fish, European sea bass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), cod (Gadus morhua) and salmon (Salmo salar), using volatile compounds by SPME/GC/MS. Journal of the Science of Food and Agriculture 92(12): 2560-2568. 

Ma, R., Liu, X., Tian, H., Han, B., Li, Y., Tang, C., Zhu, K., Li, C. & Meng, Y. 2020. Odor-active volatile compounds profile of triploid rainbow trout with different marketable sizes. Aquaculture Reports 17: 100312.

Magalhães, D.M., Borges, M., Laumann, R.A., Woodcock, C.M., Withall, D.M., Pickett, J.A., Birkett, M.A. & Blassioli-Moraes, M.C. 2018. Identification of volatile compounds involved in host location by Anthonomus grandis (Cleoptra: Curculinoidae). Frontiers in Ecology and Evolution 6: 98.

Mloston, G., Shermolovich, Y. & Heimgartner, G. 2022. Synthesis of fluorinated and fluoroalkylated heterocycles containing at least one sulfur atom via cycloaddition reactions. Materials 15(20): 7244.

Orabi, E.A. & English, A.M. 2016. Sulfur-aromatic interactions: Modeling cysteine and methionine binding to tyrosinate and histidinium ions to assess their influence on protein electron transfer. Israel Journal of Chemistry 56(9): 872-885.

Pavia, D.L., Lampman, G.M., Kriz, G.S. & Vyvyan, J.R. 2014. Introduction to Spectroscopy. 5th ed. Washington. Western Washington University.

Pratama, R.I., Rostini, I. & Rochima, E. 2018a. Amino acid profile and volatile flavour compounds of raw and steamed patin catfish (Pangasius hypophthalamus) and narrow-barred spanish mackerel (Scomberomorus commerson). In IOP Conference Series: Earth and Environmental Science 1(116): 012056.

Pratama, I.R., Rosstini, I. & Rochima, E. 2018b. Profil asam amino, asam lemak dan komponen volatil ikan     gurame segar (Osphronemus gouramy) dan kukus. Jurnal Pengolahan Hasil Perikanan Indonesia 21(2): 218-231.

Sae-Leaw, T. & Benjakul, S. 2015. Physico-chemical properties and fishy odour of gelatin from seabass (Lates calcarifer) skin stored in ice. Food Bioscience 10: 59-68.

Saraiva, C., Vasconcelos, H. & de Almeida, J.M.M.M. 2017. A chemometrics approach applied to Fourier transform infrared spectroscopy (FTIR) for monitoring the spoilage of fresh salmon (Salmo salar) stored under modified atmospheres. International Journal of Food Microbiology 241: 331-339.

Sodiq, M., Sudarmadji & Sutoyo. 2016. Pengaruh warna dan volume tempat atraktan terhadap lalat buah belimbing di Kecamatan Palang, Tuban-Jawa Timur. Plumula 5(2): 107-112.

Sun, K., Dai, Z., Hong, W., Zhao, J., Zhao, H., Luo, J. & Xie, G. 2022. Effects of maillard reaction on volatile compounds and antioxidant capacity of cat food attractant. Molecules 27(21): 7239.

Toniolo, R., Dossi, N., Svigely, R., Susmel, S., Casella, I.G. & Bontempelli, G. 2014. Amperometric sniffer for volatile amines based on paper-supported room temperature ionic liquids enabling rapid assessment of fish spoilage. Electroanalysis 26(9): 1966-1974.

Waters Corporation. 2012. Amino Acid Analysis Application Notebook. https:// www.waters.com/webassets/cms/library/ docs/720006130en.pdf

Wang, H.L., Ding, B.J., Dai, J.Q., Najarenus, T.J., Borges, R., Neto, A.M., Cahoon, E.B., Hofvander, P., Stymne, S. & Löfstedt, C.  2022. Insect pest management with sex pheromone precursors from engineered oilseed plants. Natural Sustainability 5(11): 981-990.

Xuan, G., Guo, M., Lin, H., Sui, J. & Wang, J. 2023. Study on volatile chemicals as spoilage indexes of salmon by HS-SPME-GC-MS technique during non-frozen storage. Molecules28(1): 13.

Zhou, X., Chong, Y., Ding, Y., Gu, S. & Liu, L. 2016. Determination of the effects of different washing processes on aroma characteristics in silver carp mince by MMSE-GC-MS, e-nose and sensory evaluation. Food Chemistry 207: 205-213

*Pengarang untuk surat-menyurat; email: hanifahlioe@apps.ipb.ac.id